vendredi 21 juin 2013

RT-PCR kit norovirus


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Sapovirus@ceeramTools


Catalogue References :
Kit sapovirus(assay)  KHSAV for Clinical samples (CE IVD)
Kit sapovirus(assay)  KSAV for Food and Environmental samples
Kit Quantification Standard KQSAV

SAPOVIRUS responsible for viral gastroenteritis outbreaks

Sapoviruses are non enveloped and single strand RNA viruses. These human enteric viruses are responsible for viral gastroenteritis outbreaks. They are pathogenic for all age groups of the population. These viruses are very infectious; one to ten viral particles are sufficient to get sick.

FOOD poisoning with SAPOVIRUS

Their mode of transmission is the faeco-oral route. After excretion in human stool, due to their structural properties, Sapoviruses can resist to different physical and chemical treatment. Adsorbed on particles, they can survive in different types of environment for a long period. Food samples can be contaminated via food handlers or by the environment. Due to their structural properties, Sapoviruses can survive to different food processes. The consumption of contaminated food can lead to human outbreak.

methods of choice for SAPOVIRUS detection

The detection by ELISA is not recommended due toSapoviruses  genetic diversity  and its lack of sensitivity. Molecular techniques (real time RT-PCR) are the methods of choice for SaV detection  after extraction and viral RNA purification from the sample.


CeeramTools Molecular Detection System (RT-PCR Test Kits) also available for :

MengoVirus RT-PCR Kit Rotavirus RT-PCR KitEnterorivus RT-PCR KitAstrovirus RT-PCR KitAdenovirus PCR KitHepatitis A Virus RT-PCR Kit - AichiVirus RT-PCR Kit - Circovirus Kit - Brachyspira KitCryptosporidium PCR Kit - Giardia PCR Kit - Norovirus GI RT-PCR Kit - Hepatitis E Virus RT-PCR Kit - Norovirus GII RT-PCR Kit - Norovirus GIV RT-PCR Kit

RT-PCR kits working with following rT-PCR thermal cyclers :


ABI 7300/7500 / 7900
StepOne®
StepOne® Plus
LightCycler 2.0
LightCycler 480
Rotorgene Mastercycler IQ5
CFX96-CFX384
Chromo4
RotorGene Q Stratagene Smartcycler


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